Eriodictyol-induced anti-cancer and apoptotic effects

Eriodictyol

Widespread flavanone found esp. in citrus fruits (lemon, tangerine, orange, grapefruit and pummelo) and peppermint. Glycosides also widely distributed.

Eriodictyol is found in many foods, some of which are tamarind, sacred lotus, cloud ear fungus, and common oregano.

2016 Research

Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Abstract

The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein.

References

Amin A, Hamza AA, Bajbouj K, Ashraf SS, Daoud S. Saffron: A potential candidate for a novel anticancer drug against hepatocellular carcinoma. Hepatology. 2011; 54: 857-67.

Clavin M, Gorzalczany S, Macho A, Muñoz E, Ferraro G, Acevedo C, Martino V. Anti-inflammatory activity of flavonoids from Eupatorium arnottianum. J Ethnopharmacol. 2007; 112: 58589.

Fabregat I. Dysregulation of apoptosis in hepatocellular carcinoma cells. World J Gastroenterol. 2009; 15: 513-20.

Flatt PM, Pietenpol JA. Mechanisms of cell-cycle checkpoints: At the crossroads of carcinogenesis and drug discovery. Drug Metab Rev. 2000; 32: 283-305.

George BP, Abrahamse H. A review on novel breast cancer therapies: Photodynamic therapy and plant derived agent induced cell death mechanisms. Anticancer Agents Med Chem. 2015.

Havsteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol. 1983; 32; 1141-48.

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61: 69-90.

Ley JP, Krammer G, Reinders G, Gatfield IL, Bertram HJ. Evaluation of bitter masking flavanones from Herba Santa (Eriodictyon californicum (H. and A.) Torr., Hydrophyllaceae). J Agric Food Chem. 2005; 53: 606166.

Ngandeu F, Bezabih M, Ngamga D, Tchinda AT, Ngadjui BT, Abegaz BM, Dufat H, Tillequin F.Rotenoid derivatives and other constituents of the twigs of Millettia duchesnei. Phytochemistry 2008; 69: 25863.

Pfeuty B, David-Pfeuty T, Kaneko K.Underlying principles of cell fate determination during G1 phase of the mammalian cell cycle. Cell Cycle. 2008; 7: 3246-57.

Pietenpol JA, Stewart ZA. Cell cycle checkpoint signaling: Cell cycle arrest versus apoptosis. Toxicology 2002; 181-182: 475-81.

Qazzaz ME, Raja VJ, Lim KH, Kam TS, Lee JB, Gershkovich P, Bradshaw TD. In vitro anticancer properties and biological evaluation of novel natural alkaloid jerantinine b. Cancer Lett. 2015; 370: 185-97.

Tabone M, Pellicano R. Prevention of intrahepatic hepatocarcinoma recurrence in patients with viral cirrhosis: Two potential options. Minerva Gastroenterol Dietol. 2006; 52: 47-52.

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