Skip to main content

Eriodictyol-induced anti-cancer and apoptotic effects

Eriodictyol
Widespread flavanone found esp. in citrus fruits (lemon, tangerine, orange, grapefruit and pummelo) and peppermint. Glycosides also widely distributed.

Eriodictyol is found in many foods, some of which are tamarind, sacred lotus, cloud ear fungus, and common oregano.
2016 Research
Eriodictyol-induced anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are associated with cell cycle arrest and modulation of apoptosis-related proteins

Abstract
The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein.

References

Amin A, Hamza AA, Bajbouj K, Ashraf SS, Daoud S. Saffron: A potential candidate for a novel anticancer drug against hepatocellular carcinoma. Hepatology. 2011; 54: 857-67.
Clavin M, Gorzalczany S, Macho A, Muñoz E, Ferraro G, Acevedo C, Martino V. Anti-inflammatory activity of flavonoids from Eupatorium arnottianum. J Ethnopharmacol. 2007; 112: 58589.
Fabregat I. Dysregulation of apoptosis in hepatocellular carcinoma cells. World J Gastroenterol. 2009; 15: 513-20.
Flatt PM, Pietenpol JA. Mechanisms of cell-cycle checkpoints: At the crossroads of carcinogenesis and drug discovery. Drug Metab Rev. 2000; 32: 283-305.
George BP, Abrahamse H. A review on novel breast cancer therapies: Photodynamic therapy and plant derived agent induced cell death mechanisms. Anticancer Agents Med Chem. 2015.
Havsteen B. Flavonoids, a class of natural products of high pharmacological potency. Biochem Pharmacol. 1983; 32; 1141-48.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61: 69-90.
Ley JP, Krammer G, Reinders G, Gatfield IL, Bertram HJ. Evaluation of bitter masking flavanones from Herba Santa (Eriodictyon californicum (H. and A.) Torr., Hydrophyllaceae). J Agric Food Chem. 2005; 53: 606166.
Ngandeu F, Bezabih M, Ngamga D, Tchinda AT, Ngadjui BT, Abegaz BM, Dufat H, Tillequin F.Rotenoid derivatives and other constituents of the twigs of Millettia duchesnei. Phytochemistry 2008; 69: 25863.
Pfeuty B, David-Pfeuty T, Kaneko K.Underlying principles of cell fate determination during G1 phase of the mammalian cell cycle. Cell Cycle. 2008; 7: 3246-57.
Pietenpol JA, Stewart ZA. Cell cycle checkpoint signaling: Cell cycle arrest versus apoptosis. Toxicology 2002; 181-182: 475-81.
Qazzaz ME, Raja VJ, Lim KH, Kam TS, Lee JB, Gershkovich P, Bradshaw TD. In vitro anticancer properties and biological evaluation of novel natural alkaloid jerantinine b. Cancer Lett. 2015; 370: 185-97.
Tabone M, Pellicano R. Prevention of intrahepatic hepatocarcinoma recurrence in patients with viral cirrhosis: Two potential options. Minerva Gastroenterol Dietol. 2006; 52: 47-52.


Popular posts from this blog

Microorganisms that kill cancer cells!

Microorganisms that kill cancer cells! Do you think you are just eating vegetables & Fruits?! Think again  
Read more

The Effect of Bacillus polyfermenticus against cancer cells

Bacillus polyfermenticus & Cancer 1.The prophylactic effect of probiotic Bacillus polyfermenticus KU3 against cancer cells
Read more

Mango Health Benefits

First lets Start with the Mango Concerns Latex allergy People who suffer from latex allergy have the highest susceptibility of a cross-reaction to mangoes. Also, a high intake of potassium can be pretty harmful to those that do not possess fully functional kidneys. In the case that kidneys are unable to oversee the removal of excess potassium from the body, it can be fatal. The overall eating pattern is vital in warding off diseases as well as upholding impeccable health. Try to alternate your diet rather than just concentrating on the consumption of individual foods as key to impeccable health. Urishiol The mangoes’ sap and peels contain a chemical, urushiol, also present in poison sumac and poison ivy. Urushiol may cause an allergic reaction in individuals with the highest susceptibility. Hastening of mangoes’ ripening process Some of the mango growers, or dealers, hasten the ripening of mangoes through the use of calcium carbide, and this can cause serious health c...
Read more

Broccoli Health Benefits

1.     Prevent Cancer This is probably the main benefits of broccoli that you could get. Broccoli is widely known around the world as a perfect source to prevent you from many cancers. Many studies suggest that the broccoli could be beneficial for preventing cancers because of the compounds called isothiocyanates. This isothiocyanates have been shown to affect the liver enzymes, reduce oxidative stress, stimulate the immune system, and the most important thing is, it can inhibit the development of cancers. Also, broccoli contains glucoraphanin, which will processed by the body into an anti-cancer compound called sulforaphane, which is beneficial to get rid of bacteria that can increase the risk of cancers called H. pylori. In addition to that, broccoli also will provide you a compound called indole-3-carbinol, which is an anti-carcinogen that will prevent you from various types of cancer. 2. Reduce the Possibility of Cholesterol Well, there are several r...
Read more

Enhance Your Oxygen Levels And Heal Your Body

Oxygen Levels  The German cancer researcher Dr. Paul Gerhard Seeger" demonstrated  in 1938 that in most cases cancer starts in the the jelly-like outer part of the cell, and especially in the energy-producing mitochondria. Here food fragments are normally oxidized in a series of enzymatic steps called the ‘respiratory chain’ ( Krebs Cycle) Seeger showed that in cancer cells this respiratory chain was more or less blocked, especially at the site of the important enzyme cytochrome oxidase. Without it the cell can produce energy only anaerobically like a fungal cell. This is very inefficient and the resulting overproduction of lactic acid makes the cell and the whole body (overly acidic). Dr. Seeger’s most important discovery was the certainty that certain nutrients, mainly from the vegetable kingdom, could restore cellular respiration in low-virulence cancer cells and, with this, transform them back into normal cells. In Krebs Cycle Oxygen is t...
Read more