Eriodictyol
Widespread flavanone found esp. in
citrus fruits (lemon, tangerine, orange, grapefruit and pummelo) and
peppermint. Glycosides also widely distributed.
Eriodictyol is found in many foods, some
of which are tamarind, sacred lotus, cloud ear fungus, and common oregano.
2016 Research
Eriodictyol-induced
anti-cancer and apoptotic effects in human hepatocellular carcinoma cells are
associated with cell cycle arrest and modulation of apoptosis-related proteins
Abstract
The objective of the present study was to
investigate the anti-cancer effects of eriodictyol in human hepatocellular
carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along
with evaluating its mode of action. Sulforhodamine B assay was used to evaluate
the cytotoxic effect of the compound while as fluorescence microscopy was
involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow
cytometry was used to investigate the effect of eriodictyol on cell cycle while
Western blot analysis revealed the effect on apoptosis-related protein
expressions. Results indicate that eriodictyol-induced selective and
concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12
normal liver cells were very less susceptible to its effect.
Eriodictyol-induced apoptosis related morphological changes including chromatin
condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest
in these cells. Eriodictyol led to up-regulation of Bax and PARP and
down-regulation of Bcl-2 protein.
References
Amin A, Hamza AA, Bajbouj K, Ashraf SS, Daoud S. Saffron: A
potential candidate for a novel anticancer drug against hepatocellular
carcinoma. Hepatology. 2011; 54: 857-67.
Clavin M, Gorzalczany S, Macho A, Muñoz E, Ferraro G, Acevedo C,
Martino V. Anti-inflammatory activity of flavonoids from Eupatorium
arnottianum. J Ethnopharmacol. 2007; 112: 58589.
Fabregat I. Dysregulation of apoptosis in hepatocellular
carcinoma cells. World J Gastroenterol. 2009; 15: 513-20.
Flatt PM, Pietenpol JA. Mechanisms of cell-cycle checkpoints: At
the crossroads of carcinogenesis and drug discovery. Drug Metab Rev. 2000; 32:
283-305.
George BP, Abrahamse H. A review on novel breast cancer
therapies: Photodynamic therapy and plant derived agent induced cell death
mechanisms. Anticancer Agents Med Chem. 2015.
Havsteen B. Flavonoids, a class of natural products of high
pharmacological potency. Biochem Pharmacol. 1983; 32; 1141-48.
Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global
cancer statistics. CA Cancer J Clin. 2011; 61: 69-90.
Ley JP, Krammer G, Reinders G, Gatfield IL, Bertram HJ.
Evaluation of bitter masking flavanones from Herba Santa (Eriodictyon
californicum (H. and A.) Torr., Hydrophyllaceae). J Agric Food Chem. 2005; 53:
606166.
Ngandeu F, Bezabih M, Ngamga D, Tchinda AT, Ngadjui BT, Abegaz
BM, Dufat H, Tillequin F.Rotenoid derivatives and other constituents of the
twigs of Millettia duchesnei. Phytochemistry 2008; 69: 25863.
Pfeuty B, David-Pfeuty T, Kaneko K.Underlying principles of cell
fate determination during G1 phase of the mammalian cell cycle. Cell Cycle.
2008; 7: 3246-57.
Pietenpol JA, Stewart ZA. Cell cycle checkpoint signaling: Cell
cycle arrest versus apoptosis. Toxicology 2002; 181-182: 475-81.
Qazzaz ME, Raja VJ, Lim KH, Kam TS, Lee JB, Gershkovich P,
Bradshaw TD. In vitro anticancer properties and biological evaluation of novel
natural alkaloid jerantinine b. Cancer Lett. 2015; 370: 185-97.
Tabone M, Pellicano R. Prevention of intrahepatic
hepatocarcinoma recurrence in patients with viral cirrhosis: Two potential
options. Minerva Gastroenterol Dietol. 2006; 52: 47-52.